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A dual-phase biocide to kill microbes, bacteria and fungus.

Microbes in the form of bacteria and fungus are present in all diesel fuels. Long periods of fuel storage can create ideal opportunities for microbes to grow in fuel tanks. The first indication of microbial contamination is mucous-like accumulations on fuel-filters and increased requirements for fuel-filter replacement. Microbes can only be removed from the fuel system by use of a diesel fuel biocide.

  • Dual-Phase — effective in both diesel fuel and water as recommended by major diesel engine manufacturers
  • Effective as a “quick-kill” biocide and for long-term diesel fuel maintenance
  • Kills microbes (bacteria and fungi) that grow in dispersed and free water associated with diesel fuels
  • Prevents fuel-filter plugging — helps prevent fuel system failures
  • Protects fuel system components against organic acids created by microbial growth — prevents fuel tank corrosion
  • Improves fuel performance
  • Reduces engine maintenance costs
  • Burns similarly to diesel fuel (>106,000 BTU/Gal)
  • Effective in all diesel fuels, including Ultra Low Sulfur Diesel (ULSD), biodiesel and biodiesel blends
9016-09 9/1 16-Ounce 320 Gallons 880 Gallons
9080-06 6/1 80-Ounce 1,600 Gallons 4,400 Gallons
9055 55-Gallon Drum 146,300 Gallons 387,200 Gallons


Directions for use
Use slug dosage or intermittent metering of Bio Kleen® Diesel Fuel Biocide to attain “shock-treatment” concentrations of contaminated diesel fuel tanks or for maintenance-treatment concentrations. STORE ABOVE 50º F.

SHOCK TREATMENT OF DIESEL FUEL BULK-STORAGE OR EQUIPMENT TANKS: Treat total capacity of each tank – not the amount of fuel in the tank at the time of treatment. Add 1 ounce of Bio Kleen to each 20 gallons of equipment fuel tank capacity (see treatment ratio chart on the other side). After treatment has been added, fill the tank with diesel fuel to maximum capacity so that the rising level of diesel fuel treats the entire interior walls of the tank.

MAINTENANCE TREATMENT OF DIESEL FUEL BULK-STORAGE OR EQUIPMENT TANKS : Treat total capacity of each tank at the ratio of 1 ounce of Bio Kleen to each 55 gallons of diesel fuel in the tank (see treatment ratio chart on the other side). After treatment, fill the tank to maximum capacity.

SHOCK TREATMENT AND MAINTENANCE TREATMENT: Recirculate fuel in tank for 30 minutes following treatment, if possible. Let diesel fuel set for eight hours before using to achieve maximum effectiveness. Treatment may result in additional accumulations of water and dead microbes at the bottom of the tank. If so, pump or drain tank bottoms to complete tank clean-up.

WHEN TO USE A BIOCIDE: Make visual inspections of bulk-storage tanks. Dark, slimy growth may be detected at the interface area between the diesel fuel and the water-bottom or throughout the fuel. A “rotten egg” odor may also be detected. Look for slime on interior walls of tanks and in the sump area – check water drawn from the sump area for slimy debris. Microbial growth can multiply rapidly and may form into long strings and form mats or globules. These are the most common signs of contamination and require immediate treatment.

Shock treatment may be performed to solve contamination problems, followed by maintenance treatments at regularly scheduled intervals. Or, the shock treatment may be repeated every three months. Following treatment of the tank, inspect filters frequently until plugging no longer occurs.

PRACTICE GOOD HOUSEKEEPING: Although Bio Kleen® is a dual-phase biocide and is effective in both diesel fuel and water, it is recommended that accumulated water be drained or pumped regularly from tank bottoms and that sump areas be drained. Filtering is also recommended to remove fungal debris. Inspect filters and screens regularly. A good maintenance program can reduce microbial growth and increase effectiveness of shock and maintenance treatments.  


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Click link below for MSDS
Bio Kleen® Diesel Fuel Biocide

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